First molecular evidence of Borrelia burgdorferi sensu lato in goats, sheep, cattle and camels in Tunisia

Borrelia burgdorferi sensu lato (s.l.) are tick-transmitted spirochaetes of veterinary and human importance. Molecular
epidemiology data on ruminants are still lacking in most countries of the world. Therefore, the aim of this study was to
estimate the rate of B. burgdorferi s.l. infection in ruminants from Tunisia. A total of 1,021 ruminants (303 goats, 260 sheep,
232 cattle and 226 camels) from different bioclimatic areas in Tunisia were investigated for the presence of B. burgdorferi
s.l. DNA in blood by real time PCR. Prevalence rates were 30.4% (92/303) in goats, 6.2% (16/260) in sheep, 1.3% (3/232) in
cattle, and 1.8% (4/226) in camels. Only tick species belonging to Rhipicephalus and Hyalomma genera were found on the
investigated animals. In small ruminants, the prevalence of B. burgdorferi s.l. varied significantly according to localities and
farms. Goats located in humid areas were statistically more infected than those located in sub-humid areas. Prevalence
rates varied significantly according to age and breed in sheep, and age and tick infestation in goats. This study provides
the first insight into the presence of B. burgdorferi s.l. DNA in ruminants in Tunisia, and demonstrates that host species such
as goats and sheep may play an important role in natural Lyme disease cycles in this country.