Publication Date:
2012
Short description:
FTIR nanobiosensors for Escherichia coli detection / Mura, S., Greppi, G., Marongiu, M.L., Roggero, P.P., Ravindranath, S.p., Mauer, L.j., Schibeci, N., Perria, F., Massimo, P., Innocenzi, P., Joseph Irudayaraj, J.. - In: BEILSTEIN JOURNAL OF NANOTECHNOLOGY. - ISSN 2190-4286. - 3:(2012), pp. 485-492. [10.3762/bjnano.3.55]
abstract:
Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal
health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast
and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we
report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl)
triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties
monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective
method for the effective screening of water samples.
health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast
and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we
report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl)
triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties
monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective
method for the effective screening of water samples.
Iris type:
1.1 Articolo in rivista
List of contributors:
Mura, Stefania; Greppi, Gianfranco; Marongiu, Maria Laura; Roggero, Pier Paolo; Ravindranath, Sp; Mauer, Lj; Schibeci, N; Perria, F; Massimo, Piccinini7; Innocenzi, Plinio; Joseph Irudayaraj, J.
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