Data di Pubblicazione:
2011
Citazione:
The testing season affects red deer skinfold increase in response to phytohaemagglutinin / Fernández de Mera*, Ig; Jaroso, R; Martín Hernando, Mp; Queiros, J; Carta, Tania; Ortiz, Ja; Vicente, J; Gortázar, C.. - In: PREVENTIVE VETERINARY MEDICINE. - ISSN 0167-5877. - 100:(2011), pp. 79-83. [10.1016/j.prevetmed.2011.02.011]
Abstract:
Red deer(Cervus elaphus) haveapronouncedseasonalityintheirphysiology.Theaimof
this studywastoassesstheeffectoftheseasononreddeerresponsivenesstoskintesting
with thephytohaemagglutinin(PHA)mitogen.Studysubjectsincluded270farmedadult
red deer(19stagsand251hinds).TheskintestingwascarriedoutbetweenJanuary2009
and August2010.Theanimalswereinjectedintradermallywitha0.1mlvolumecontaining
250g ofPHAdilutedinphosphatebufferedsaline.Theskinfoldthicknesswasmeasured
immediately priortoinjectionand72hafteradministration,alwaysbythesamepersonand
with threerepeatspermeasurement.Singleeffectsofsexandtimeonskintestresponsive-
ness weresignificant(p < 0.001)aswellastheirinteraction(p < 0.001).Inwinter(January),
and consideringtheaverageoftwoyears,theskinfoldincreaseinresponsetotheintra-
dermal injectionof250g PHAwas2.1timeslargerinstagsand1.4timesinhindsthan
in summer(August).Whilestagshad1.3timeslargerresponsesthanhindsinwinter,the
inverse occurredinsummer,with1.1timeslargerresponsesinhinds.Wealsoevidenced
a limitedinter-annualvariationofskinfoldincreaseinresponsetoPHAinreddeer.These
findings haveimportantconsequencesregardingtheinterpretationofskintestresultsin
the ante-mortemdiagnosisoftuberculosisandparatuberculosis,atleastindeer.
this studywastoassesstheeffectoftheseasononreddeerresponsivenesstoskintesting
with thephytohaemagglutinin(PHA)mitogen.Studysubjectsincluded270farmedadult
red deer(19stagsand251hinds).TheskintestingwascarriedoutbetweenJanuary2009
and August2010.Theanimalswereinjectedintradermallywitha0.1mlvolumecontaining
250g ofPHAdilutedinphosphatebufferedsaline.Theskinfoldthicknesswasmeasured
immediately priortoinjectionand72hafteradministration,alwaysbythesamepersonand
with threerepeatspermeasurement.Singleeffectsofsexandtimeonskintestresponsive-
ness weresignificant(p < 0.001)aswellastheirinteraction(p < 0.001).Inwinter(January),
and consideringtheaverageoftwoyears,theskinfoldincreaseinresponsetotheintra-
dermal injectionof250g PHAwas2.1timeslargerinstagsand1.4timesinhindsthan
in summer(August).Whilestagshad1.3timeslargerresponsesthanhindsinwinter,the
inverse occurredinsummer,with1.1timeslargerresponsesinhinds.Wealsoevidenced
a limitedinter-annualvariationofskinfoldincreaseinresponsetoPHAinreddeer.These
findings haveimportantconsequencesregardingtheinterpretationofskintestresultsin
the ante-mortemdiagnosisoftuberculosisandparatuberculosis,atleastindeer.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Cellular immunity; Cervus elaphus; Mitogen
Elenco autori:
Fernández de Mera*, Ig; Jaroso, R; Martín Hernando, Mp; Queiros, J; Carta, Tania; Ortiz, Ja; Vicente, J; Gortázar, C.
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