Data di Pubblicazione:
2007
Citazione:
Preliminary survey on Ralstonia solanacearum strains isolated from tomato in Italy / Loreti, S; Fiori, Mario; De Simone, D; Falchi, G; Gallelli, A; Schiaffino, A.. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - (2007), pp. S45-S46. (Intervento presentato al convegno XIV congresso Nazionale SiPav tenutosi a Perugia nel 18 - 21 settembre).
Abstract:
Tomato wilting caused by Ralstonia solanacearum (RS) to
tomato cultivars Arawak, Cuore di Bue and Ikram growing in
greenhouses in Southern Sardinia was observed in February and
April 2007. Tomato plants were grown in the soil and in soil-less
culture in five greenhouses. The percentage of affected plants was
up to 70%. Isolations from symptomatic plants and from water
used for irrigation onto SMSA and TZCA media gave fluid white
colonies with a red centre. Pathogenicity tests were done by inoculating
six isolates each on 9 plants of tomato, eggplant and tobacco.
Only tomato and eggplant plants wilted within two weeks
and died; no symptoms were observed in tobacco and in control plants. Isolates obtained originally and from artificially inoculated
plants were characterized by biochemical and physiological tests,
BIOLOGTM system and IFAS. FAME analysis, performed on two
isolates, confirmed the similarity with RS. PCR with primers
OLY-1 and Y-2, specific for RS, gave the expected band of 288
bp. Digestion of PCR products with AvaII restriction endonuclease
gave the same pattern as the reference strain. Rep-PCR and
SDS-PAGE of whole-cell proteins confirmed the similarity of our
isolates with RS reference strain. Furthermore, partial 16S rDNA
gene sequences of several isolates were compared with known sequences
of RS and their similarity was 100%. Sequences were deposited
in GenBank (Accession numbers from AM690474 to
AM690480). In conclusion, it was ascertained that the bacteria
isolated from tomato in Sardinia have the same characteristics of
race 3 of R. solanacearum.
tomato cultivars Arawak, Cuore di Bue and Ikram growing in
greenhouses in Southern Sardinia was observed in February and
April 2007. Tomato plants were grown in the soil and in soil-less
culture in five greenhouses. The percentage of affected plants was
up to 70%. Isolations from symptomatic plants and from water
used for irrigation onto SMSA and TZCA media gave fluid white
colonies with a red centre. Pathogenicity tests were done by inoculating
six isolates each on 9 plants of tomato, eggplant and tobacco.
Only tomato and eggplant plants wilted within two weeks
and died; no symptoms were observed in tobacco and in control plants. Isolates obtained originally and from artificially inoculated
plants were characterized by biochemical and physiological tests,
BIOLOGTM system and IFAS. FAME analysis, performed on two
isolates, confirmed the similarity with RS. PCR with primers
OLY-1 and Y-2, specific for RS, gave the expected band of 288
bp. Digestion of PCR products with AvaII restriction endonuclease
gave the same pattern as the reference strain. Rep-PCR and
SDS-PAGE of whole-cell proteins confirmed the similarity of our
isolates with RS reference strain. Furthermore, partial 16S rDNA
gene sequences of several isolates were compared with known sequences
of RS and their similarity was 100%. Sequences were deposited
in GenBank (Accession numbers from AM690474 to
AM690480). In conclusion, it was ascertained that the bacteria
isolated from tomato in Sardinia have the same characteristics of
race 3 of R. solanacearum.
Tipologia CRIS:
4.3 Poster
Elenco autori:
Loreti, S; Fiori, Mario; De Simone, D; Falchi, G; Gallelli, A; Schiaffino, A.
Link alla scheda completa:
Titolo del libro:
Journal of Plant Pathology
Pubblicato in: