Molecular characterization of Biscogniauxia mediterranea (De Not.) O. Kuntze strains isolated from declining trees of Quercus suber L. in Sardinia
Contributo in Atti di convegno
Data di Pubblicazione:
2002
Citazione:
Molecular characterization of Biscogniauxia mediterranea (De Not.) O. Kuntze strains isolated from declining trees of Quercus suber L. in Sardinia / Schiaffino, A. FRANCESCHINI A.; Maddau, Lucia; Serra, Salvatorica. - In: IOBC/WPRS BULLETIN. - ISSN 1027-3115. - 25:5(2002), pp. 37-40. (Intervento presentato al convegno Working group Integrated Protection in Oak Forests tenutosi a Oeiras - Lisbonne (Portugal) nel 1-4 octobre 2001).
Abstract:
A collection of Sardinian isolates oi Biscogniauxia mediterranea (De Not.) O. Kuntze was
analysed by means of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction),
using twenty arbitrary 10-mer primers, in order to detect the genetic variation of the population. Five
out of twenty OPA primers were particularly effective in detecting polymorphism. The dendrogram,
obtained on the basis of these data by using UPGMA method and Pearson coefficient, showed a high
level of genetic variability among the isolates of B. mediterranea analysed.
analysed by means of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction),
using twenty arbitrary 10-mer primers, in order to detect the genetic variation of the population. Five
out of twenty OPA primers were particularly effective in detecting polymorphism. The dendrogram,
obtained on the basis of these data by using UPGMA method and Pearson coefficient, showed a high
level of genetic variability among the isolates of B. mediterranea analysed.
Tipologia CRIS:
4.1 Contributo in Atti di convegno
Keywords:
Biscogniauxia mediterranea; Quercus suber; RAPD-PCR; polymorphism
Elenco autori:
Schiaffino, A. FRANCESCHINI A.; Maddau, Lucia; Serra, Salvatorica
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