Characterization of exon 14 and intron 5 of the SREBP-1 gene in mediterranean italian buffaloes

SREBP‐1 (Sterol Regulatory Element Binding Protein) is a transcription factor that regulates the
expression levels of some genes and plays a central role in energy homeostasis. The aim of the
present study was to screen for single nucleotide polymorphisms (SNPs) or
insertions/deletions in the exon 14 and intron 5 of the SREBP‐1 gene in Mediterranean Italian
Buffaloes. Twenty multiparous buffalo cows were randomly chosen from one farm located in
Sardinia. From each buffalo cow a blood sample was taken to perform DNA extraction and
PCR‐SSCP analysis of the fragments above. Twenty PCR products for each fragment were
sequenced in both directions, then compared and aligned with sequence of Bos taurus
(GenBank AAFC03008483). The comparison between the sequence of exon 14 and intron 5
from buffalo with that of the cattle showed homology of 96%. Our data showed no variation in
exon 14 and intron 5 of the SREBP‐1 gene in Mediterranean Buffalo contrarily to that found in
cattle. However, to extend the knowledge about SREBP‐1 gene, in Mediterranean Italian
buffaloes, would be necessary to enlarge the number of samples and to study other parts of
this gene.