Hydroxyapatite-Fluoride Toothpastes on Caries Activity: A Triple-Blind Randomized Clinical Trial

The study aimed to evaluate the remineralizing effect of hydroxyapatite and fluoride containing toothpastes (HAF’s) on active caries lesions compared to a fluoridated standard toothpaste
in pre/schoolchildren. A total of 610 children (4-5 and 6-7 years old) were enrolled. Four toothpastes, 2 containing fluoride-substituted hydroxyapatite (HAF) (1000 and 1450 ppmF) and magnesium-, strontium-, carbonate-substituted hydroxyapatite, in a chitosan matrix and 2 Mono
fluoridated toothpastes (NaMFP) (1000 and 1450 ppmF), were randomly administered for 24
months. The children were instructed to brush for 2 minutes 3 times/day. Caries activities, by
clinical surface features, were recorded at baseline and 12- and 24-month follow-ups. A perprotocol analysis was adopted, thus excluding children lost to follow-up. Overall, 518 children
completed the trial. Baseline comparisons revealed no significant differences in primary teeth
caries rates between HAF and NaMFP groups, both for enamel and dentinal lesions. By the
end of the study, the HAF group exhibited a statistically significant reduction in enamel
lesions compared to the NaMFP group (P < .01). Of the 40 partially active lesions at baseline in
the HAF group, 13 were inactive at the 2-year follow-up. Of the active lesions in the HAF group
(n = 78) at baseline, nearly 3-quarters (n = 58) were inactive at the follow-up. The difference
between the 2 groups (HAF vs NaMFP) in terms of change of status in primary dentition (active
at baseline and inactive at follow-up) was statistically significant (P = .04). Regarding dentinal
lesions, both groups presented similar percentages of inactive lesions that were filled at the
end of follow-up (P = .08). However, the HAF group demonstrated a higher number of inactivated lesions compared to the NaMFP group. The toothpaste containing biomimetic hydroxyapatite and fluoride may be better for children with active caries lesion in primary dentition.

2024 The Authors. Published by Elsevier Inc. on behalf of FDI World Dental Federation. This
is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)