PROFILO DEI GENI CHE CODIFICANO PER LE ENTEROTOSSINE E LA TSST-1 IN S. AUREUS ISOLATO DA LATTE DI CAPRA ALLA PRODUZIONE
Contributo in Atti di convegno
Data di Pubblicazione:
2005
Citazione:
PROFILO DEI GENI CHE CODIFICANO PER LE ENTEROTOSSINE E LA TSST-1
IN S. AUREUS ISOLATO DA LATTE DI CAPRA ALLA PRODUZIONE / Scarano, Christian; Mureddu, A.; Virdis, S.; Pilo, A. L.; Mazzette, R.; DE SANTIS, Enrico Pietro Luigi; Bes, M.. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - (2005), pp. 407-408. (Intervento presentato al convegno LIX Congresso SISVET tenutosi a Viareggio (LU) nel 21 - 24 settembre 2005).
Abstract:
Thirty S. aureus strains isolated from goat’s bulk milk were analysed by
multiplex PCR for staphylococcal enterotoxins (SEs) and TSST-1 encoding genes. All strains
were tested for gyrA, agr I-IV alleles, SEs genes (sea-e, seh, sek, sel, sem, seo) and TSST-1
(tst). SEA-E production was also tested using ELISA. All strains possessed gyrA and agr
alleles. Fourteen out of thirty strains harboured either one or more toxin genes and the most
common pattern that coexisted in S. aureus was sec, sel and tst (11 strains). Genes seb and seh
were not found. Eight different profiles were observed based on the association of agr alleles,
SEs and tst. Further test conducted by ELISA given concordances with PCR in eleven out of
fourteen enterotoxigenic strains.
multiplex PCR for staphylococcal enterotoxins (SEs) and TSST-1 encoding genes. All strains
were tested for gyrA, agr I-IV alleles, SEs genes (sea-e, seh, sek, sel, sem, seo) and TSST-1
(tst). SEA-E production was also tested using ELISA. All strains possessed gyrA and agr
alleles. Fourteen out of thirty strains harboured either one or more toxin genes and the most
common pattern that coexisted in S. aureus was sec, sel and tst (11 strains). Genes seb and seh
were not found. Eight different profiles were observed based on the association of agr alleles,
SEs and tst. Further test conducted by ELISA given concordances with PCR in eleven out of
fourteen enterotoxigenic strains.
Tipologia CRIS:
4.1 Contributo in Atti di convegno
Keywords:
S. aureus; enterotossine; latte di capra
Elenco autori:
Scarano, Christian; Mureddu, A.; Virdis, S.; Pilo, A. L.; Mazzette, R.; DE SANTIS, Enrico Pietro Luigi; Bes, M.
Link alla scheda completa:
Titolo del libro:
LIX Convegno Nazionale Società Italiana Delle Scienze Veterinarie
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