Population structure of theMonocelis lineata(Proseriata, Monocelididae) species complex assessed by phylogenetic analysis of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene
Articolo
Data di Pubblicazione:
2009
Citazione:
Population structure of theMonocelis lineata(Proseriata, Monocelididae) species complex assessed by phylogenetic analysis of the mitochondrial
Cytochrome c Oxidase subunit I (COI) gene / Sanna, Daria; Curini Galletti, Marco; Lai, Tiziana; Francalacci, Paolo; Casu, Marco. - In: GENETICS AND MOLECULAR BIOLOGY. - ISSN 1415-4757. - 32:4(2009), pp. 864-867.
Abstract:
Monocelis lineateconsists of a complex of sibling species, widespread in the Mediterranean and Atlantic Ocean.
Previous genetic analysis placed in evidence at least four sibling species. Nevertheless, this research was not conclusive
enough to fully resolve the complex or to infer the phylogeny/phylogeography of the group. We designed specific
primers aiming at obtaining partial sequences of the mtDNA gene Cytochrome c Oxidase subunit I (COI) ofM.
lineate, and have identified 25 different haplotypes in 32 analyzed individuals. The dendrogram generated by Neighbor-
Joining analysis confirmed the differentiation between Atlantic and Mediterranean siblings, as well as the occurrence
of at least two Mediterranean sibling species. Thus validated, the method here presented appears as a
valuable tool in population genetics and biodiversity surveys on theMonocelis lineatecomplex.
Previous genetic analysis placed in evidence at least four sibling species. Nevertheless, this research was not conclusive
enough to fully resolve the complex or to infer the phylogeny/phylogeography of the group. We designed specific
primers aiming at obtaining partial sequences of the mtDNA gene Cytochrome c Oxidase subunit I (COI) ofM.
lineate, and have identified 25 different haplotypes in 32 analyzed individuals. The dendrogram generated by Neighbor-
Joining analysis confirmed the differentiation between Atlantic and Mediterranean siblings, as well as the occurrence
of at least two Mediterranean sibling species. Thus validated, the method here presented appears as a
valuable tool in population genetics and biodiversity surveys on theMonocelis lineatecomplex.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Microturbellaria; sequencing; sibling species; phylogeography; phylogeny
Elenco autori:
Sanna, Daria; Curini Galletti, Marco; Lai, Tiziana; Francalacci, Paolo; Casu, Marco
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