A New automatic system of cell colony counting

The counting process of cell colonies is always a long
and laborious process that is dependent on the judgment and ability
of the operator. The judgment of the operator in counting can vary in
relation to fatigue. Moreover, since this activity is time consuming it
can limit the usable number of dishes for each experiment. For these
purposes, it is necessary that an automatic system of cell colony
counting is used. This article introduces a new automatic system of
counting based on the elaboration of the digital images of cellular
colonies grown on petri dishes. This system is mainly based on the
algorithms of region-growing for the recognition of the regions of
interest (ROI) in the image and a Sanger neural net for the
characterization of such regions. The better final classification is
supplied from a Feed-Forward Neural Net (FF-NN) and confronted
with the K-Nearest Neighbour (K-NN) and a Linear Discriminative
Function (LDF). The preliminary results are shown.