Minisatellites in Saccharomyces cerevsiae cell wall genes: a new way towards wine strain identification
Articolo
Data di Pubblicazione:
2004
Citazione:
Minisatellites in Saccharomyces cerevsiae cell wall genes: a new way towards wine strain identification / Marinangeli, P.; Angelozzi, D.; Ciani, M.; Clementi, F.; Mannazzu, Ilaria Maria. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 4:(2004), pp. 427-435. [10.1016/S1567-1356(03)00172-7]
Abstract:
With the aim of developing new tools for the characterisation of wine yeasts, by means of databases available on-line we scanned the
genome of Saccharomyces cerevisiae in search of potentially polymorphic targets. As we have previously observed for SED1, we found
that other genes coding for cell wall proteins contain minisatellite-like sequences. A polymerase chain reaction (PCR) survey of SED1 and
three of these others, namely AGA1, DAN4 and HSP150, in a population of wild S. cerevisiae demonstrated that these genes are highly
polymorphic in length and represent a sink of unexplored genetic variability. The primer pairs designed on the gene open reading frames
yield stable and repeatable amplification profiles that show a level of resolution that allows the clear discriminate between different
strains. These can therefore be utilised for PCR-based typing of S. cerevisiae.
genome of Saccharomyces cerevisiae in search of potentially polymorphic targets. As we have previously observed for SED1, we found
that other genes coding for cell wall proteins contain minisatellite-like sequences. A polymerase chain reaction (PCR) survey of SED1 and
three of these others, namely AGA1, DAN4 and HSP150, in a population of wild S. cerevisiae demonstrated that these genes are highly
polymorphic in length and represent a sink of unexplored genetic variability. The primer pairs designed on the gene open reading frames
yield stable and repeatable amplification profiles that show a level of resolution that allows the clear discriminate between different
strains. These can therefore be utilised for PCR-based typing of S. cerevisiae.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Minisatellite ; Cell wall gene; PCR ¢ngerprinting; Saccharomyces cerevisiae
Elenco autori:
Marinangeli, P.; Angelozzi, D.; Ciani, M.; Clementi, F.; Mannazzu, Ilaria Maria
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