Data di Pubblicazione:
2019
Citazione:
Lessons from human umbilical cord: gender differences in stem cells from Wharton’s jelly / Balzano, Francesca; Bellu, Emanuela; Basoli, Valentina; Giudici, Silvia Dei; Santaniello, Sara; Cruciani, Sara; Facchin, Federica; Oggiano, Annalisa; Capobianco, Giampiero; Dessole, Francesco; Ventura, Carlo; Dessole, Salvatore; Maioli, Margherita. - In: EUROPEAN JOURNAL OF OBSTETRICS, GYNECOLOGY, AND REPRODUCTIVE BIOLOGY. - ISSN 0301-2115. - 234(2019), pp. 143-148. [10.1016/j.ejogrb.2018.12.028]
Abstract:
Objective To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity.
Design Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord
Setting University hospital
Patient (s) umbilical cords (n=12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women
Intervention we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1).
Mean outcome measure WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR
Results expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences.
Conclusion our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.
Design Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord
Setting University hospital
Patient (s) umbilical cords (n=12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women
Intervention we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1).
Mean outcome measure WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR
Results expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences.
Conclusion our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Stem cells; Wharton jelly; Gender; epigenetics; pluripotency
Elenco autori:
Balzano, Francesca; Bellu, Emanuela; Basoli, Valentina; Giudici, Silvia Dei; Santaniello, Sara; Cruciani, Sara; Facchin, Federica; Oggiano, Annalisa; Capobianco, Giampiero; Dessole, Francesco; Ventura, Carlo; Dessole, Salvatore; Maioli, Margherita
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