Influence of Culture Media on the Motility and Viability of Cryopreserved Stallion Spermatozoa
Abstract
Publication Date:
2002
Short description:
Influence of Culture Media on the Motility and Viability
of Cryopreserved Stallion Spermatozoa / Leoni, Giovanni Giuseppe; Rosati, Irma; Berlinguer, F; Ledda, Sergio; Pintus, P; Bogliolo, Luisa; Cherchi, R; Naitana, S.. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 37:(2002), pp. 241-241. (Intervento presentato al convegno 6th Annual Meeting of the European Society for Domestic Animal Reproduction (ESDAR) tenutosi a Parma nel 12-14 settembre 2002).
abstract:
Thawed cryopreserved stallion semen was washed in PBS+0.3%BSA
at 900 g for 5¢and cultured in 35 mm petri dishes in two different
media: SOF+0.3%BSA (SOF) and TCM199+0.3% BSA (TCM)
supplemented or not with 1500 UI/ml SOD. At 0, 3, 6, 9, 24 and 48 h
of culture motility, viability and acrosome status were evaluated with
propidium iodide and FITC-PSA. Results indicate that mass motility,
measured as a 0–10 grade scale by three different operators, was higher
in SOF than in TCM (7.3 vs. 3.7, 6 vs. 3, 4.3 vs. 2.3, 2 vs. 1, 0.7 vs. 0 at
3, 6, 9, 12, 24 and 48 h of culture respectively). SOD supplementation
increased spermatozoa motility compared to the basic media (SOF:
8.7, 8.3, 6.3, 3, 2.39; TCM: 5.3, 4, 2.7,1, 0 for the same times). Cell
viability was higher (p < 0:01) in SOF compared to TCM (3h:50.0 and
45, 6h : 54.8% vs. 32.3%, 9h : 39.1% vs. 15.5%, 24h : 14.5% vs.
2.0%, 48h : 5.5% vs. 0 for SOF and TCM, respectively). The viability
rate was increased (p < 0:05) in TCM+SOD (3h : 45.1 vs. 54.1,
6h : 32.3 vs. 44.1, 9h : 15.5 vs. 37.5 and 24h : 2 vs. 10) but not in
SOF+SOD. The rate of live spermatozoa with intact acrosome
membrane was 52.5% at thawing and decreased significantly during
the first 3 h of culture in both media (17.1% and 15.4% for SOD and
TCM respectively, p < 0:01). SOD supplementation to both media did
not influence acrosome integrity during the culture. SOF appeared as
more efficient than TCM to preserve stallion sperm viability and
motility during in vitro culture.
at 900 g for 5¢and cultured in 35 mm petri dishes in two different
media: SOF+0.3%BSA (SOF) and TCM199+0.3% BSA (TCM)
supplemented or not with 1500 UI/ml SOD. At 0, 3, 6, 9, 24 and 48 h
of culture motility, viability and acrosome status were evaluated with
propidium iodide and FITC-PSA. Results indicate that mass motility,
measured as a 0–10 grade scale by three different operators, was higher
in SOF than in TCM (7.3 vs. 3.7, 6 vs. 3, 4.3 vs. 2.3, 2 vs. 1, 0.7 vs. 0 at
3, 6, 9, 12, 24 and 48 h of culture respectively). SOD supplementation
increased spermatozoa motility compared to the basic media (SOF:
8.7, 8.3, 6.3, 3, 2.39; TCM: 5.3, 4, 2.7,1, 0 for the same times). Cell
viability was higher (p < 0:01) in SOF compared to TCM (3h:50.0 and
45, 6h : 54.8% vs. 32.3%, 9h : 39.1% vs. 15.5%, 24h : 14.5% vs.
2.0%, 48h : 5.5% vs. 0 for SOF and TCM, respectively). The viability
rate was increased (p < 0:05) in TCM+SOD (3h : 45.1 vs. 54.1,
6h : 32.3 vs. 44.1, 9h : 15.5 vs. 37.5 and 24h : 2 vs. 10) but not in
SOF+SOD. The rate of live spermatozoa with intact acrosome
membrane was 52.5% at thawing and decreased significantly during
the first 3 h of culture in both media (17.1% and 15.4% for SOD and
TCM respectively, p < 0:01). SOD supplementation to both media did
not influence acrosome integrity during the culture. SOF appeared as
more efficient than TCM to preserve stallion sperm viability and
motility during in vitro culture.
Iris type:
4.2 Abstract in Atti di convegno
List of contributors:
Leoni, Giovanni Giuseppe; Rosati, Irma; Berlinguer, F; Ledda, Sergio; Pintus, P; Bogliolo, Luisa; Cherchi, R; Naitana, S.
Book title:
Proceedings of the 6th Annual Meeting of the European Society for Domestic Animal Reproduction (ESDAR)
Published in: