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CARATTERIZZAZIONE GENOTIPICA E STUDIO DI CLONALITÀ DI CEPPI DI S. AUREUS ISOLATI DA LATTE DI CAPRA ALLA PRODUZIONE

Conference Paper
Publication Date:
2006
Short description:
CARATTERIZZAZIONE GENOTIPICA E STUDIO DI CLONALITÀ DI CEPPI DI S. AUREUS ISOLATI DA LATTE DI CAPRA ALLA PRODUZIONE / Scarano, Christian; Corgiolu, G.; Virdis, S.; Marongiu, P.; DE SANTIS, Enrico Pietro Luigi; Bes, M.. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - (2006), pp. 451-452. (Intervento presentato al convegno LX Convegno Nazionale Società Italiana Delle Scienze Veterinarie tenutosi a Terrasini (PA) nel 27/30 Settembre 2006).
abstract:
Thirty S. aureus strains isolated from goat’s bulk milk were analysed by multiplex PCR for the following genes: gyrA (gyrase); agr I-IV alleles (accessory gene regulator); sea-e, seh, sek, sel, sem, seo (enterotoxins genes); tst (TSST-1); eta, etb, etd (exfoliatins); lukF-PV and lukS-PV (Panton-Valentine leukocidin); lukE and lukD (lukE-lukD leukocidin); lukM (lukM leukocidin); hlg, hlgv and hlb (γ, γ variant and ß hemolysines); edin (epidermal cell differentiation inhibitor); mecA (methicillin resistance determinant). All strains were also analysed by PFGE and MLST. Genes agr III, sec, sel, tst, LukE-lukD, lukM, hlgv and hlb were found in majority strains, while agr IV, seb, seh, mecA, eta, etb, etd, lukF-PV and lukS-PV were not found. PFGE and MLST subdivided the strains respectively in 14 pulsotypes, 7 groups of similarity and 7 classes.
Iris type:
4.1 Contributo in Atti di convegno
Keywords:
S. aureus; enterotoxins; goat’s milk; Multiplex PCR; PFGE; MLST
List of contributors:
Scarano, Christian; Corgiolu, G.; Virdis, S.; Marongiu, P.; DE SANTIS, Enrico Pietro Luigi; Bes, M.
Authors of the University:
DE SANTIS Enrico Pietro Luigi
SCARANO Christian
Handle:
https://iris.uniss.it/handle/11388/73723
Book title:
Atti LX Congresso S.I.S.Vet.
Published in:
ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE
Journal
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