Publication Date:
2015
Short description:
Molecular typing of XDR Acinetobacter baumannii strains in an Italian ICU / Piana, A.F., Palmieri, A., Deidda, S., Mura, E., Are, B.M., Rubino, S., Sanna, S., Calaresu, E., Cocuzza, C., Sotgiu, G., Mura, I.I.. - In: EPIDEMIOLOGIA E PREVENZIONE. - ISSN 1120-9763. - 39:4 (suppl. 1)(2015), pp. 129-133.
abstract:
Abstract
Objective. To investigate the antimicrobial susceptibility and clonal relationship of Acinetobacter baumannii strains isolated in an Italian ICU.
Design. Epidemiological, observational, retrospective, longitudinal study.
Setting and participants. The ICU of the University Hospital of Sassari, Italy.
Main outcome measures. Pulsed Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing (MLST) were used to eval- uate the genomic features of the isolated strains.
Results. Drug susceptibility testing for all isolated strains showed the same resistance pattern, characterized by resistance to the most important antibiotics, with the only exception of colistin. PFGE showed a very poor between-strain variability; three dis- tinct clusters, 11, 4, and 1 isolates in size, were identified (Dice’s coefficient: 92.11%). MLST showed that all isolated strains be- longed to sequence type 2 (ST2). All isolates collected from the environment and the human samples were positive for the fol- lowing genes: blaOXA-23, blaOXA-51-like, blaVIM-like, blaIMP-like, andISAba1; however, blaOXA-24-like, blaOXA-58-like, and blaNDM-like were not detected.
Conclusions. The survey identified XDR strains belonging to the same cell clone, confirming the wide circulation and environ- mental persistence of this microorganism.
Objective. To investigate the antimicrobial susceptibility and clonal relationship of Acinetobacter baumannii strains isolated in an Italian ICU.
Design. Epidemiological, observational, retrospective, longitudinal study.
Setting and participants. The ICU of the University Hospital of Sassari, Italy.
Main outcome measures. Pulsed Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing (MLST) were used to eval- uate the genomic features of the isolated strains.
Results. Drug susceptibility testing for all isolated strains showed the same resistance pattern, characterized by resistance to the most important antibiotics, with the only exception of colistin. PFGE showed a very poor between-strain variability; three dis- tinct clusters, 11, 4, and 1 isolates in size, were identified (Dice’s coefficient: 92.11%). MLST showed that all isolated strains be- longed to sequence type 2 (ST2). All isolates collected from the environment and the human samples were positive for the fol- lowing genes: blaOXA-23, blaOXA-51-like, blaVIM-like, blaIMP-like, andISAba1; however, blaOXA-24-like, blaOXA-58-like, and blaNDM-like were not detected.
Conclusions. The survey identified XDR strains belonging to the same cell clone, confirming the wide circulation and environ- mental persistence of this microorganism.
Iris type:
1.1 Articolo in rivista
Keywords:
antimìcrobial susceptibility ; Molecular typing
List of contributors:
Piana, Andrea Fausto; Palmieri, Alessandra; Deidda, Silvia; Mura, Erica; Are, Bianca Maria; Rubino, Salvatore; Sanna, Silvana; Calaresu, Enrico; Cocuzza, Clementina; Sotgiu, Giovanni; Mura, Ida Iolanda
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