Full-length protein extraction protocols and gel-based downstream applications in formalin-fixed tissue proteomics
Chapter
Publication Date:
2015
Short description:
Full-length protein extraction protocols and gel-based downstream applications in formalin-fixed tissue proteomics / Tanca, A., Uzzau, S., Addis, M.f.. - 1295:(2015), pp. 117-134. [10.1007/978-1-4939-2550-6_11]
abstract:
Archival formalin-fi xed, paraffi n-embedded (FFPE) tissue repositories and their associated clinical information
can represent a valuable resource for tissue proteomics. In order to make these tissues available for
protein biomarker discovery and validation studies, dedicated sample preparation procedures overcoming
the intermolecular cross-links introduced by formalin need to be implemented. This chapter describes a
full-length protein extraction protocol optimized for downstream gel-based proteomics applications.
Using the procedures detailed here, SDS-PAGE, western immunoblotting, GeLC-MS/MS, 2D-PAGE,
and 2D-DIGE can be carried out on FFPE tissues. Technical tips, critical aspects, and drawbacks of the
method are presented and discussed.
can represent a valuable resource for tissue proteomics. In order to make these tissues available for
protein biomarker discovery and validation studies, dedicated sample preparation procedures overcoming
the intermolecular cross-links introduced by formalin need to be implemented. This chapter describes a
full-length protein extraction protocol optimized for downstream gel-based proteomics applications.
Using the procedures detailed here, SDS-PAGE, western immunoblotting, GeLC-MS/MS, 2D-PAGE,
and 2D-DIGE can be carried out on FFPE tissues. Technical tips, critical aspects, and drawbacks of the
method are presented and discussed.
Iris type:
2.1 Contributo in volume (Capitolo o Saggio)
List of contributors:
Tanca, Alessandro; Uzzau, Sergio; Addis, Mf
Book title:
Proteomic Profiling: Methods and Protocols
Published in: