Phenotypic and genetic characterization of Salmonella enterica subsp. enterica serovar Typhimurium isolated from pigs in Rio Grande do Sul, Brazil
Academic Article
Publication Date:
2007
Short description:
Phenotypic and genetic characterization of Salmonella enterica subsp. enterica serovar Typhimurium isolated from pigs in Rio Grande do Sul, Brazil / Bessa, Mc; Michael, Gb; Canu, N; Canal, Cw; Cardoso, M; Rabsch, W; Rubino, Salvatore. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 83:(2007), pp. 302-310.
abstract:
This study aimed to investigate the relatedness of porcine Salmonella enterica subsp. enterica (S.) serovar Typhimurium strains isolated
in Southern Brazil. Sixty-six isolates from pigs belonging to three commercial companies were submitted to phage typing, XbaImacrorestriction
(PFGE), IS200 hybridization, rep-PCR, antimicrobial susceptibility testing, and PCR assay targeting the spvR region.
All strains presented a unique rep-PCR pattern and 63 strains had a common IS200 profile. One pulse-type (XA) was the most prevalent
(39/66 strains) and included strains of phage types DT177, DT192, DT194 and RDNC. The spvR region was detected in three strains,
which harboured plasmids of 90 kb. High rates of tetracycline, sulfonamide and streptomycin resistance were found. Isolates from farms
located in different geographic regions but associated to the same commercial companies clustered together and presented a common
resistance profile. Results suggested that clonal groups of S. Typhimurium are present in pig commercial companies in Southern Brazil.
2007 Elsevier Ltd. All rights reserved.
in Southern Brazil. Sixty-six isolates from pigs belonging to three commercial companies were submitted to phage typing, XbaImacrorestriction
(PFGE), IS200 hybridization, rep-PCR, antimicrobial susceptibility testing, and PCR assay targeting the spvR region.
All strains presented a unique rep-PCR pattern and 63 strains had a common IS200 profile. One pulse-type (XA) was the most prevalent
(39/66 strains) and included strains of phage types DT177, DT192, DT194 and RDNC. The spvR region was detected in three strains,
which harboured plasmids of 90 kb. High rates of tetracycline, sulfonamide and streptomycin resistance were found. Isolates from farms
located in different geographic regions but associated to the same commercial companies clustered together and presented a common
resistance profile. Results suggested that clonal groups of S. Typhimurium are present in pig commercial companies in Southern Brazil.
2007 Elsevier Ltd. All rights reserved.
Iris type:
1.1 Articolo in rivista
List of contributors:
Bessa, Mc; Michael, Gb; Canu, N; Canal, Cw; Cardoso, M; Rabsch, W; Rubino, Salvatore
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