Immunomodulation of fucosyl-lactose and lacto-N-fucopentaose on mononuclear cells from multiple sclerosis and healthy subjects
Academic Article
Publication Date:
2006
Short description:
Immunomodulation of fucosyl-lactose and lacto-N-fucopentaose
on mononuclear cells from multiple sclerosis and healthy subjects / Sotgiu, Stefano; Rosati, Giulio; Arru, Giannina; Fois, Maria Laura; Musumeci, Maria; Sanna, Alessandra; Musumeci, Salvatore. - 2:2(2006), pp. 114-120.
abstract:
The 1,2-fucosyl-oligosaccharides, and among these the 2’-fucosyl-lactose (2’-FL) and lacto-N-fucopentaose (LNFP)-I, are quantitatively the most represented
oligosaccharides of human milk. They are also seen to represent an important
immune device to prevent nursing infants from severe infectious diarrhoea.
Recent evidences show that the appearance of 2’-FL and LNFP-I in human colostrums
is synchronised with the macrophage inhibition and that LNFP-III induces
a Th2 response from the mouse peripheral immune system. Since mannosyl-fucosyl
receptors are described on the macrophage surface, all these evidences allow
us to investigate on the possible immune function of human 2’-FL and LNFP-I in
vitro on LPS-activated mononuclear cells (MNC) from 12 patients with multiple
sclerosis (MS) and 20 matched health controls (HC). We found that 2’-FL and
LNFP-I significantly decrease, to a different extent, the MNC proliferation from
both HC and MS patients, in a linear and dose-dependent manner. 2’-FL and LNFPI
also reduce the production of IL-12 and IFN-γ, particularly in MS patients as
compared to HC (p=0.01 and p<0.001, respectively), while increasing that of IL-
10. The overall immunomodulatory effect of 2’-FL and LNFP I here presented may
represent a future therapeutic option for the abnormal immune response found in
some monocyte-mediated diseases.
oligosaccharides of human milk. They are also seen to represent an important
immune device to prevent nursing infants from severe infectious diarrhoea.
Recent evidences show that the appearance of 2’-FL and LNFP-I in human colostrums
is synchronised with the macrophage inhibition and that LNFP-III induces
a Th2 response from the mouse peripheral immune system. Since mannosyl-fucosyl
receptors are described on the macrophage surface, all these evidences allow
us to investigate on the possible immune function of human 2’-FL and LNFP-I in
vitro on LPS-activated mononuclear cells (MNC) from 12 patients with multiple
sclerosis (MS) and 20 matched health controls (HC). We found that 2’-FL and
LNFP-I significantly decrease, to a different extent, the MNC proliferation from
both HC and MS patients, in a linear and dose-dependent manner. 2’-FL and LNFPI
also reduce the production of IL-12 and IFN-γ, particularly in MS patients as
compared to HC (p=0.01 and p<0.001, respectively), while increasing that of IL-
10. The overall immunomodulatory effect of 2’-FL and LNFP I here presented may
represent a future therapeutic option for the abnormal immune response found in
some monocyte-mediated diseases.
Iris type:
1.1 Articolo in rivista
Keywords:
Mononuclear cells; 2-fucosyl-lactose; lacto-N-fucopentaose I; multiple sclerosis
List of contributors: