Publication Date:
2001
Short description:
Genomic instability in chronic viral hepatitis and hepatocellular carcinoma / Dore, M.P., Realdi, G., Mura, D., Onida, A., Massarelli, G., Dettori, G.L., Graham, D.y., Sepulveda, A.R.. - In: HUMAN PATHOLOGY. - ISSN 0046-8177. - 32:7(2001), pp. 698-703. [10.1053/hupa.2001.25593]
abstract:
Chronic hepatitis may progress to cirrhosis and hepatocellular
carcinoma (HCC). Progressive accumulation of mutations and
genomic instability in chronic viral hepatitis might flag an increased
risk of HCC development. Genomic instability at dinucleotide microsatellite
loci in chromosomes 2,13,and 17 and at 2 mononucleotide
repeat loci was examined in liver tissues from 41 patients,
including 30 without HCC (18 patients with chronic hepatitis and 12
with cirrhosis) and 11 with HCC. Genomic instability was detected in
51% of the 41 cases. Allelic imbalance at informative dinucleotide
loci occurred in 37% of the cases. In 14 cases (34%),allelic imbalance
was detected in chronic hepatitis or cirrhosis without HCC. Allelic
imbalance at the chromosome 13 locus was detected in 50% of the
cases of chronic hepatitis C. Allelic imbalance at the TP53 chromosome
locus and/or at the chromosome 13 locus was significantly
more frequent than alterations at the chromosome 2 locus (P .026).
Low-level microsatellite instability was found in 20% of all cases
examined and high-level microsatellite instability in 3 patients (7.5%),
including 2 cases of chronic hepatitis and 1 case of cirrhosis. Our
results show that allelic imbalance occurs frequently in hepatitisrelated
HCC as well as in chronic hepatitis in patients without HCC.
Allelic imbalance at the D13S170 chromosome 13 locus (13q31.2)
occurs frequently in chronic hepatitis,suggesting that genomic alterations
affecting the long arm of chromosome 13 might be used to
monitor the natural progression of chronic hepatitis–associated liver
carcinogenesis. HUM PATHOL 32:698-703. Copyright © 2001 by W.B.
Saunders Company
Key words: genomic instability,chronic hepatitis,cirrhosis,
hepatocellular carcinoma.
Abbreviations: HCC,hepatocellular carcinoma; HBV,hepatitis B
virus; HCV,hepatitis C virus; AFB,aflatoxin B; MSI,microsatellite
instability; AI,allelic imbalance; LOH,loss of heterozygosity;
HNPCC,hereditary nonpolyposis colorectal cancer.
carcinoma (HCC). Progressive accumulation of mutations and
genomic instability in chronic viral hepatitis might flag an increased
risk of HCC development. Genomic instability at dinucleotide microsatellite
loci in chromosomes 2,13,and 17 and at 2 mononucleotide
repeat loci was examined in liver tissues from 41 patients,
including 30 without HCC (18 patients with chronic hepatitis and 12
with cirrhosis) and 11 with HCC. Genomic instability was detected in
51% of the 41 cases. Allelic imbalance at informative dinucleotide
loci occurred in 37% of the cases. In 14 cases (34%),allelic imbalance
was detected in chronic hepatitis or cirrhosis without HCC. Allelic
imbalance at the chromosome 13 locus was detected in 50% of the
cases of chronic hepatitis C. Allelic imbalance at the TP53 chromosome
locus and/or at the chromosome 13 locus was significantly
more frequent than alterations at the chromosome 2 locus (P .026).
Low-level microsatellite instability was found in 20% of all cases
examined and high-level microsatellite instability in 3 patients (7.5%),
including 2 cases of chronic hepatitis and 1 case of cirrhosis. Our
results show that allelic imbalance occurs frequently in hepatitisrelated
HCC as well as in chronic hepatitis in patients without HCC.
Allelic imbalance at the D13S170 chromosome 13 locus (13q31.2)
occurs frequently in chronic hepatitis,suggesting that genomic alterations
affecting the long arm of chromosome 13 might be used to
monitor the natural progression of chronic hepatitis–associated liver
carcinogenesis. HUM PATHOL 32:698-703. Copyright © 2001 by W.B.
Saunders Company
Key words: genomic instability,chronic hepatitis,cirrhosis,
hepatocellular carcinoma.
Abbreviations: HCC,hepatocellular carcinoma; HBV,hepatitis B
virus; HCV,hepatitis C virus; AFB,aflatoxin B; MSI,microsatellite
instability; AI,allelic imbalance; LOH,loss of heterozygosity;
HNPCC,hereditary nonpolyposis colorectal cancer.
Iris type:
1.1 Articolo in rivista
List of contributors:
Dore, Maria Pina; Realdi, G; Mura, D; Onida, A; Massarelli, Giovannino; Dettori, Giuseppe Lorenzo; Graham, Dy; Sepulveda, A. R.
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