Detection, virulence genes and antimicrobial resistance of Yersinia enterocolitica in sheep and goat raw milk
Academic Article
Publication Date:
2021
Short description:
Detection, virulence genes and antimicrobial resistance of Yersinia enterocolitica in sheep and goat raw milk / Piras, F., Spanu, C., Sanna, R., Siddi, G., Mocci, A.M., Demontis, M., Meloni, M.P., Spanu, V., De Santis, E.P.L., Scarano, C.. - In: INTERNATIONAL DAIRY JOURNAL. - ISSN 0958-6946. - 117:(2021), p. 105011. [10.1016/j.idairyj.2021.105011]
abstract:
The aim of this study was to evaluate the prevalence of Yersinia enterocolitica in sheep and goat raw milk
in Sardinia (Italy). Overall, 738 samples from bulk milk tanks were collected: 507 raw sheep milk samples
and 231 raw goat milk samples. Y. enterocolitica was detected from 24/507 (4.8%) raw sheep milk and 24/
231 (10.4%) goat milk samples. Prevalence was significantly higher (P < 0.01) in goat milk than in sheep
milk. Biotyping of Y. enterocolitica isolates identified only biotype 1A. The most common virulence genes
were investigated: ystB gene was found in 42/48 (87.5%) and inv in 40/48 (83.3%) strains. All the isolates
were susceptible to ciprofloxacin, nalidixic acid, sulphonamide, tetracycline and trimethoprim. Five AMR
profiles were identified showing resistance to amoxicillin-clavulanic acid, ticarcillin, cephalexin, cefoxitin
and ceftriaxone. Resistance to third generation cephalosporins is of particular concern as they are recommended
for therapeutic use in case of human illness.
in Sardinia (Italy). Overall, 738 samples from bulk milk tanks were collected: 507 raw sheep milk samples
and 231 raw goat milk samples. Y. enterocolitica was detected from 24/507 (4.8%) raw sheep milk and 24/
231 (10.4%) goat milk samples. Prevalence was significantly higher (P < 0.01) in goat milk than in sheep
milk. Biotyping of Y. enterocolitica isolates identified only biotype 1A. The most common virulence genes
were investigated: ystB gene was found in 42/48 (87.5%) and inv in 40/48 (83.3%) strains. All the isolates
were susceptible to ciprofloxacin, nalidixic acid, sulphonamide, tetracycline and trimethoprim. Five AMR
profiles were identified showing resistance to amoxicillin-clavulanic acid, ticarcillin, cephalexin, cefoxitin
and ceftriaxone. Resistance to third generation cephalosporins is of particular concern as they are recommended
for therapeutic use in case of human illness.
Iris type:
1.1 Articolo in rivista
List of contributors:
Piras, Francesca; Spanu, Carlo; Sanna, Rita; Siddi, Giuliana; Mocci, Anna Maria; Demontis, Mariella; Meloni, Maria Pina; Spanu, Vincenzo; De Santis, Enrico Pietro Luigi; Scarano, Christian
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