Natalizumab inhibits the expression of human endogenous retroviruses of the W family in multiple sclerosis patients: a longitudinal cohort study.
Articolo
Data di Pubblicazione:
2014
Citazione:
Natalizumab inhibits the expression of human endogenous retroviruses of the W family in multiple sclerosis patients: a longitudinal cohort study / Arru, Giannina; Leoni, S; Pugliatti, M; Mei, A; Serra, Caterina; Delogu, Lucia Gemma; Manetti, Roberto; Dolei, Antonina; Sotgiu, Stefano; Mameli, Giuseppe. - In: MULTIPLE SCLEROSIS. - ISSN 1352-4585. - Feb;20(2):174-82:2(2014), pp. 174-182. [10.1177/1352458513494957]
Abstract:
Abstract
BACKGROUND:
Several viruses were reported as co-factors triggering the pathogenesis of multiple sclerosis (MS), including the endogenous retroviruses of the HERV-W family, that were also proposed as biomarkers of disease progression and therapy outcome.
OBJECTIVE:
The objective of this article is to clarify whether in MS patients treatment with natalizumab has effects on MSRV/syncytin-1/HERV-W expression and the possible relationship with disease outcome.
METHODS:
Peripheral blood mononuclear cells were collected from 22 patients with relapsing-remitting disease, at entry and after three, six and 12 months of treatment with natalizumab. The cell subpopulations and the expression of MSRVenv/syncytin-1/HERV-Wenv were analyzed by flow cytometry and by discriminatory env-specific RT-PCR assays.
RESULTS:
By flow cytometry the relative amounts of T, NK and monocyte subpopulations were shown to remain fairly constant. A relative increase of B lymphocytes was observed at three to six months (p = 0.033). The MSRVenv and syncitin-1 transcripts were reduced at six to 12 months of therapy (p = 0.0001). Accordingly, at month 12, the plasma-membrane levels of the HERV-Wenv protein were reduced (p = 0.0001). B cells, NK and monocytes but not T cells expressed the HERV-Wenv protein. None of the patients relapsed during therapy.
CONCLUSION:
Effective therapy with natalizumab downregulates MSRV/syncytin-1/HERV-W expression.
BACKGROUND:
Several viruses were reported as co-factors triggering the pathogenesis of multiple sclerosis (MS), including the endogenous retroviruses of the HERV-W family, that were also proposed as biomarkers of disease progression and therapy outcome.
OBJECTIVE:
The objective of this article is to clarify whether in MS patients treatment with natalizumab has effects on MSRV/syncytin-1/HERV-W expression and the possible relationship with disease outcome.
METHODS:
Peripheral blood mononuclear cells were collected from 22 patients with relapsing-remitting disease, at entry and after three, six and 12 months of treatment with natalizumab. The cell subpopulations and the expression of MSRVenv/syncytin-1/HERV-Wenv were analyzed by flow cytometry and by discriminatory env-specific RT-PCR assays.
RESULTS:
By flow cytometry the relative amounts of T, NK and monocyte subpopulations were shown to remain fairly constant. A relative increase of B lymphocytes was observed at three to six months (p = 0.033). The MSRVenv and syncitin-1 transcripts were reduced at six to 12 months of therapy (p = 0.0001). Accordingly, at month 12, the plasma-membrane levels of the HERV-Wenv protein were reduced (p = 0.0001). B cells, NK and monocytes but not T cells expressed the HERV-Wenv protein. None of the patients relapsed during therapy.
CONCLUSION:
Effective therapy with natalizumab downregulates MSRV/syncytin-1/HERV-W expression.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
natalizumab; multiple sclerosis therapy; MSRV/syncytin-1/HERV-W expression
Elenco autori:
Arru, Giannina; Leoni, S; Pugliatti, M; Mei, A; Serra, Caterina; Delogu, Lucia Gemma; Manetti, Roberto; Dolei, Antonina; Sotgiu, Stefano; Mameli, Giuseppe
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