Data di Pubblicazione:
2015
Citazione:
Structural evidences for a secondary gold binding site in the hydrophobic box of Lysozyme / Ferraro, G; Massai, L; Messori, L; Cinellu, Maria Agostina; Merlino, A.. - In: BIOMETALS. - ISSN 0966-0844. - 28:(2015), pp. 745-754.
Abstract:
A new crystal structure is reported here for
the adduct formed in the reaction between NH4
[Au(Sac)2], AuSac2, a cytotoxic homoleptic
gold(I) complex with the saccharinate ligand, and the
model protein hen egg white lysozyme. To produce this
adduct, AuSac2 breaks down and releases both
saccharinate ligands. The resulting Au(I) ions bind
the protein to ND1 and NE2 atoms of His15 but also to
SD atom of the zero-solvent accessible Met105 side
chain, which is located in the protein hydrophobic box.
The unexpected existence of this secondary
gold(I) binding site is confirmed by spectroscopic
and spectrometric measurements in solution.
the adduct formed in the reaction between NH4
[Au(Sac)2], AuSac2, a cytotoxic homoleptic
gold(I) complex with the saccharinate ligand, and the
model protein hen egg white lysozyme. To produce this
adduct, AuSac2 breaks down and releases both
saccharinate ligands. The resulting Au(I) ions bind
the protein to ND1 and NE2 atoms of His15 but also to
SD atom of the zero-solvent accessible Met105 side
chain, which is located in the protein hydrophobic box.
The unexpected existence of this secondary
gold(I) binding site is confirmed by spectroscopic
and spectrometric measurements in solution.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
gold-based drugs; protein-ligand interactions; mechanism of action
Elenco autori:
Ferraro, G; Massai, L; Messori, L; Cinellu, Maria Agostina; Merlino, A.
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