Ni(II) interaction with a histidine-rich region of the human TLR4 protein involved in the activation of nickel contact allergy
Abstract
Data di Pubblicazione:
2017
Citazione:
Ni(II) interaction with a histidine-rich region of the human TLR4 protein involved in the activation of nickel contact allergy / Zoroddu, Maria Antonietta; Peana, Massimiliano Francesco; Medici, Serenella; Pelucelli, Alessio; Simula, Giancarlo; Zdyb, Karolina; Gumienna Kontecka, Elzbieta. - In: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY. - ISSN 0946-672X. - 44:s1(2017), pp. 42-42.
Abstract:
Ni(II) stimulates innate immunity via the direct binding to
human toll like receptor 4 (hTLR4), the bacterial lipopolysaccharide
receptor. This interaction is specific for humans and causes
nickel contact allergy. The protein sequence of hTLR4 revealed that
the ectodomain, the region supposed to coordinate the metal ions,
contains a histidine-rich motif that is not conserved among all organisms.
To elucidate the role of each histidine on the protein–nickel
binding, we investigated the Ni(II) interaction with the model peptide
NH2-FQHSNRKQMSERSVFRSRRNRIYRDISHTHTR-COO-, which
encompasses the sequence 429–460 of hTLR4. Potentiometric,
UV–Vis, nuclear magnetic resonance and circular dichroism measurements
demonstrate that the non-conserved histidine in the
ectodomain cooperates in metal coordination and consequently
enables the activation of the molecular mechanism involved in
nickel hypersensitivity reaction.
human toll like receptor 4 (hTLR4), the bacterial lipopolysaccharide
receptor. This interaction is specific for humans and causes
nickel contact allergy. The protein sequence of hTLR4 revealed that
the ectodomain, the region supposed to coordinate the metal ions,
contains a histidine-rich motif that is not conserved among all organisms.
To elucidate the role of each histidine on the protein–nickel
binding, we investigated the Ni(II) interaction with the model peptide
NH2-FQHSNRKQMSERSVFRSRRNRIYRDISHTHTR-COO-, which
encompasses the sequence 429–460 of hTLR4. Potentiometric,
UV–Vis, nuclear magnetic resonance and circular dichroism measurements
demonstrate that the non-conserved histidine in the
ectodomain cooperates in metal coordination and consequently
enables the activation of the molecular mechanism involved in
nickel hypersensitivity reaction.
Tipologia CRIS:
1.5 Abstract in rivista
Keywords:
nickel allergy, TLR4, NMR
Elenco autori:
Zoroddu, Maria Antonietta; Peana, Massimiliano Francesco; Medici, Serenella; Pelucelli, Alessio; Simula, Giancarlo; Zdyb, Karolina; Gumienna Kontecka, Elzbieta
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