Stir Bar Sorptive Extraction coupled with GC/MS Applied to Honey: Optimization Method and Comparative Study with Head Space Extraction Techniques
Academic Article
Publication Date:
2017
Short description:
Stir Bar Sorptive Extraction coupled with GC/MS Applied to Honey: Optimization Method and Comparative Study with Head Space Extraction Techniques / Petretto, G.L., Urgeghe, P.P., Mascia, I., Fadda, C., Rourke, J.P., Pintore, G.A.M.. - In: EUROPEAN FOOD RESEARCH AND TECHNOLOGY. - ISSN 1438-2385. - 243:5(2017), pp. 735-741. [10.1007/s00217-016-2787-9]
abstract:
The stir bar sorptive extraction (SBSE) techniques,
coupled with gas chromatography–mass spectrometry,
were successfully applied to the study of Eucalyptus
honey’s for the determination of volatile organic compounds
(VOCs). An optimization of the extraction method
was carried out and the variables, NaCl concentration (used
as matrix modifier), and the concentration of honey solution
were studied targeting the whole VOCs composition.
After the evaluation of the experiments, the best condition
for the extraction of honey volatile components was
2 mol/L of NaCl and the more concentrated honey solution
(0.5 g of honey per mL of water). Additionally, the results
were compared with those obtained by two headspace (HS)
techniques, namely solid-phase microextraction (SPME)
and dynamic headspace (DHS). SBSE volatiles differ
qualitatively and quantitatively from those obtained by
the SPME and DHS methods. In any event, the chemical
composition of Eucalyptus honey volatiles extracted by all
three techniques shows the presence of some typical floral
markers. Our results confirm a general trend reported in the
literature, which show the higher sensitivity of SBSE in the
extraction of less volatile compounds in comparison with
HS methods
coupled with gas chromatography–mass spectrometry,
were successfully applied to the study of Eucalyptus
honey’s for the determination of volatile organic compounds
(VOCs). An optimization of the extraction method
was carried out and the variables, NaCl concentration (used
as matrix modifier), and the concentration of honey solution
were studied targeting the whole VOCs composition.
After the evaluation of the experiments, the best condition
for the extraction of honey volatile components was
2 mol/L of NaCl and the more concentrated honey solution
(0.5 g of honey per mL of water). Additionally, the results
were compared with those obtained by two headspace (HS)
techniques, namely solid-phase microextraction (SPME)
and dynamic headspace (DHS). SBSE volatiles differ
qualitatively and quantitatively from those obtained by
the SPME and DHS methods. In any event, the chemical
composition of Eucalyptus honey volatiles extracted by all
three techniques shows the presence of some typical floral
markers. Our results confirm a general trend reported in the
literature, which show the higher sensitivity of SBSE in the
extraction of less volatile compounds in comparison with
HS methods
Iris type:
1.1 Articolo in rivista
Keywords:
DHS, SBSE, SPME, Eucalyptus honey,
Floral marker, VOCs
List of contributors:
Petretto, Giacomo Luigi; Urgeghe, Pietro Paolo; Mascia, Ilaria; Fadda, Costantino; Rourke, J. P.; Pintore, Giorgio Antonio Mario
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