Quantitative assay for bradykinin in rat plasma by liquid chromatography coupled to tandem mass spectrometry.
Articolo
Data di Pubblicazione:
2011
Citazione:
Quantitative assay for bradykinin in rat plasma by liquid chromatography coupled to tandem mass spectrometry / Baralla, E.; Nieddu, N.; Boatto, Gianpiero; Varoni, Maria Vittoria; Palomba, D.; Demontis, Maria Piera; Pasciu, V.; Anania, Vittorio Domenico. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 54:3(2011), pp. 557-561. [10.1016/j.jpba.2010.09.041]
Abstract:
An assay to quantify bradykinin in rat plasma has been developed and validated, using liquid chromatography
coupled to tandem mass spectrometry (LC–MS/MS). Sar-d-Phe8-des-Arg9-bradykinin was used as
internal standard. Aprotinin was added to rat plasma to inhibit the activity of proteinases. Recoveries for
solid-phase extraction (SPE) on Strata X reversed phase were greater than 80%. Multiple reaction monitoring
(MRM) on a triple quadrupole mass spectrometer equipped with an electrospray source (ESI),
operating in the positive ion-mode, was used for detection. The assay was validated and stability was
explored. Bradykinin (10–500 ng/mL) was quantified with accuracy values (% RE) below 10% and intraand
inter-day precisions (% RSD) below 12 and 16%, respectively, for all concentrations. The method was
successfully applied to several plasma samples from low levels kallikrein rats (LKRs) compared with
normal kallikrein rats (NKRs).
coupled to tandem mass spectrometry (LC–MS/MS). Sar-d-Phe8-des-Arg9-bradykinin was used as
internal standard. Aprotinin was added to rat plasma to inhibit the activity of proteinases. Recoveries for
solid-phase extraction (SPE) on Strata X reversed phase were greater than 80%. Multiple reaction monitoring
(MRM) on a triple quadrupole mass spectrometer equipped with an electrospray source (ESI),
operating in the positive ion-mode, was used for detection. The assay was validated and stability was
explored. Bradykinin (10–500 ng/mL) was quantified with accuracy values (% RE) below 10% and intraand
inter-day precisions (% RSD) below 12 and 16%, respectively, for all concentrations. The method was
successfully applied to several plasma samples from low levels kallikrein rats (LKRs) compared with
normal kallikrein rats (NKRs).
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Bradykinin; Rat plasma; LC–MS/MS; Kallikrein; SPE
Elenco autori:
Baralla, E.; Nieddu, N.; Boatto, Gianpiero; Varoni, Maria Vittoria; Palomba, D.; Demontis, Maria Piera; Pasciu, V.; Anania, Vittorio Domenico
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