Data di Pubblicazione:
2009
Citazione:
Solubilization methods and reference 2-DE map of cow milk fat globules / Bianchi, L; Puglia, M; Landi, C; Matteoni, S; Perini, D; Armini, A; Verani, M; Trombetta, C; Soldani, P; Roncada, P; Greppi, Gianfranco; Pallini, V; Bini, L.. - In: JOURNAL OF PROTEOMICS. - ISSN 1874-3919. - Jul 21;72:(2009), pp. 853-864.
Abstract:
Milk fat globules (MFGs) are secretory vesicles assembled and secreted by mammary
epithelial cells during lactation. They consist of fat globules surrounded by a lipid bilayer
membrane which is derived from the apical membrane of the lactating cells. MFGs contain,
besides lipids, proteins from the apical plasma membrane and from the cytoplasmatic
material. Their peculiar vesicle nature makes them a suitable and easily available source of
biological material in monitoring the physiopathological state of the mammary gland.
Unfortunately, the conspicuous lipidic component of MFGs consistently limits protein
extraction and purification for MFG proteomic investigations.
This work deals with the development of a suitable procedure for
epithelial cells during lactation. They consist of fat globules surrounded by a lipid bilayer
membrane which is derived from the apical membrane of the lactating cells. MFGs contain,
besides lipids, proteins from the apical plasma membrane and from the cytoplasmatic
material. Their peculiar vesicle nature makes them a suitable and easily available source of
biological material in monitoring the physiopathological state of the mammary gland.
Unfortunately, the conspicuous lipidic component of MFGs consistently limits protein
extraction and purification for MFG proteomic investigations.
This work deals with the development of a suitable procedure for
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
milk ; fat ; proteomic
Elenco autori:
Bianchi, L; Puglia, M; Landi, C; Matteoni, S; Perini, D; Armini, A; Verani, M; Trombetta, C; Soldani, P; Roncada, P; Greppi, Gianfranco; Pallini, V; Bini, L.
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