VALUTAZIONE DELLA RESISTENZA AGLI ANTIBIOTICI IN S. AUREUS ISOLATI DA FORMAGGI AL LATTE CRUDO DI PECORA
Conference Paper
Publication Date:
2010
Short description:
VALUTAZIONE DELLA RESISTENZA AGLI ANTIBIOTICI IN S. AUREUS ISOLATI DA FORMAGGI AL LATTE CRUDO DI PECORA / Spanu, V; Virdis, S; Scarano, Christian; Cossu, F; DE SANTIS, Enrico Pietro Luigi; Cosseddu, A. M.. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - 1:3(2010), pp. 419-421. (Intervento presentato al convegno LXIII Convegno Nazionale Società Italiana Delle Scienze Veterinarie tenutosi a udine nel 16-18 settembre).
abstract:
In vitro activities of 16 antibiotics were tested against 36 S. aureus (SA) isolated from raw sheep’s milk
curds taken from 6 factories. The MICs of 16 antibiotics was determined by the broth microdilution method (CLSI). All
36 isolates were analyzed to detect accessory gene regulator agrI-IV and genes encoding resistance for methicillin
(mecA), erythromycin (ermA), penicillin (blaZ) and vancomycin (vanA-B). The isolates were also analyzed to investigate
PFGE type diversity. SA strains showed resistance to ampicillin (36.1%), penicillin (33.3%), tetracycline (11.1%) and
cloxacillin (2.8%), whereas they were susceptible (94.4%) to 12 out of 16 tested antimicrobials. In the strains which
were resistant according to broth microdilution method corrispondent encoding genes were usually detected. The overall
susceptibility of the strains to the oxacillin, vancomycin and erythromycin is confirmed by the absence of the mecA,
vanA-B and ermA. The PFGE showed that 32 strains belonged to 10 different pulsotype (P1-P10) while 4 were untypable.
curds taken from 6 factories. The MICs of 16 antibiotics was determined by the broth microdilution method (CLSI). All
36 isolates were analyzed to detect accessory gene regulator agrI-IV and genes encoding resistance for methicillin
(mecA), erythromycin (ermA), penicillin (blaZ) and vancomycin (vanA-B). The isolates were also analyzed to investigate
PFGE type diversity. SA strains showed resistance to ampicillin (36.1%), penicillin (33.3%), tetracycline (11.1%) and
cloxacillin (2.8%), whereas they were susceptible (94.4%) to 12 out of 16 tested antimicrobials. In the strains which
were resistant according to broth microdilution method corrispondent encoding genes were usually detected. The overall
susceptibility of the strains to the oxacillin, vancomycin and erythromycin is confirmed by the absence of the mecA,
vanA-B and ermA. The PFGE showed that 32 strains belonged to 10 different pulsotype (P1-P10) while 4 were untypable.
Iris type:
4.1 Contributo in Atti di convegno
Keywords:
S. aureus; sheep cheese; antibiotic resistance; MIC; genes
List of contributors:
Spanu, V; Virdis, S; Scarano, Christian; Cossu, F; DE SANTIS, Enrico Pietro Luigi; Cosseddu, A. M.
Book title:
Atti LXIII Convegno Nazionale Società Italiana Delle Scienze Veterinarie, Udine (UD), 16-18 Settembre
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