SVILUPPO DI UNA PCR MULTIPLEX PER L’IDENTIFICAZIONE E SIEROTIPIZZAZIONE DI L. MONOCYTOGENES ISOLATA IN CASEIFICI OVINI
Conference Paper
Publication Date:
2006
Short description:
SVILUPPO DI UNA PCR MULTIPLEX PER L’IDENTIFICAZIONE E SIEROTIPIZZAZIONE DI L. MONOCYTOGENES ISOLATA IN CASEIFICI OVINI / DE SANTIS, Enrico Pietro Luigi; Pilo, Al; Cosseddu, Am; Canu, Na; Scarano, Christian; Marongiu, P.. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - (2006), pp. 461-462. (Intervento presentato al convegno LX Congresso Annuale della Società Italiana delle Scienze Veterinarie (SISVet) tenutosi a Terrasini (PA) nel 27-30 Settembre 2006).
abstract:
A new multiplex PCR for identification and serotyping of L. monocytogenes
(L.m.) was developed and tested on reference strains of L.m.(5), L. innocua, L. welshimeri, L.
seeligeri, and L. ivanovii. PCR primers were designed as previously described from iap gene
fragment for identification and variable regions of the L.m. genome for serotyping in order to
classify the strains into five groups [1/2a(3a), 1/2b, 1/2c(3c), 4b(d,e), and 4a/c]. The multiplex
PCR reproducibility was also tested on 28 strains isolated from sheep’s cheese-processing
plants. The results of the PCR multiplex agreed with those of the conventional identification and
serotyping agglutination methods. The strains isolated from sheep’s dairy plant belonged to 1/2a
(17), 4b (8), and 1/2b (3) serotypes.
(L.m.) was developed and tested on reference strains of L.m.(5), L. innocua, L. welshimeri, L.
seeligeri, and L. ivanovii. PCR primers were designed as previously described from iap gene
fragment for identification and variable regions of the L.m. genome for serotyping in order to
classify the strains into five groups [1/2a(3a), 1/2b, 1/2c(3c), 4b(d,e), and 4a/c]. The multiplex
PCR reproducibility was also tested on 28 strains isolated from sheep’s cheese-processing
plants. The results of the PCR multiplex agreed with those of the conventional identification and
serotyping agglutination methods. The strains isolated from sheep’s dairy plant belonged to 1/2a
(17), 4b (8), and 1/2b (3) serotypes.
Iris type:
4.1 Contributo in Atti di convegno
Keywords:
L. monocytogenes; Multiplex PCR; serotypes; sheep’s cheese-processing plants
List of contributors:
DE SANTIS, Enrico Pietro Luigi; Pilo, Al; Cosseddu, Am; Canu, Na; Scarano, Christian; Marongiu, P.
Book title:
LX Convegno della Società Italiana delle Scienze Veterinarie
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