Quali-quantitative analysis of urinary glycosaminoglycans for monitoring glomerular inflammatory activity

Objective. A 2-year follow-up study was carried out in patients with IgA nephropathy (IgAN) in order to verify the possible
use of quali-quantitative analysis of urinary glycosaminoglycans (GAGs) as a prognostic index of disease and for drug
treatment monitoring. Material and methods. Ten patients with IgAN were evaluated at four time points: baseline, and 6, 9
and 24 months later. GAGs were isolated from 24-h urine using ion-exchange chromatography on diethylaminoethyl
Sephacel, and concentrations were expressed as milligrams of hexuronate per gram of creatinine. GAG composition was
determined by cellulose acetate electrophoresis and expressed as relative percentages by means of densitometric scanning of
Alcian Blue-stained strips. Results. The relative content of total low-sulphated chondroitin sulphate species decreased
significantly during the study period compared to baseline, whereas the relative percentages of heparan sulphate and
chondroitin sulphate increased significantly. Moreover, a significant correlation was noted between the relative contents of
urinary GAGs, renal function and inflammation indexes. Conclusions. It is likely that the excretion of various types of GAGs
may be related to different glomerular pathophysiological conditions. Therefore, the determination of urinary GAG
composition may represent a reliable indicator of disease activity.