LD50 and repellents effects of different essential oils from Argentina wild plant species on Varroa destructor mite

The repellent and acaricidal effects of some essential oils from the most typical wild plant species of northern Patagonia, Argentina, on Varroa destructor Anderson & Trueman were evaluated using a complete exposure test. Honey bees, Apis mellifera L., and mites (Þve specimens of each per dish) were introduced in petri dishes having different oil concentrations (from 0.1 to 2μl per cage). Survival of bees and mites was registered after 24, 48, and 72 h. An attraction/repellence test was performed using a wax tube impregnated with essential oil and another tube containing wax only. The lowest LD50 values for mites were registered for Acantholippia seriphioides (A. Gray) Mold. (1.27 μl per cage) and Schinus molle L. (2.6μl per cage) after 24 h, and for Wedelia glauca (Ortega) O. Hoffm. ex Hicken (0.5μl per cage) and A. seriphioides (1.09 μl per cage) after 72 h of treatment. The oil with the highest selectivity ratio (A. mellifera LD50/V. destructor LD50) was the one extracted from S. molle (>16). Oils of Lippia junelliana (Mold.) Troncoso, Minthostachys mollis (HBK) Grieseb., and Lippia turbinata Grieseb. mixed with wax had repellent properties. None of the oils tested had attractive effects on Varroa mites.