Data di Pubblicazione:
2005
Citazione:
Detection ofMycobacterium aviumsubsp.paratuberculosis(MAP) in animals and human tissue / Rocca, Stefano; Leoni, Antonio; Sechi, Leonardo Antonio; Farigu, Serafina; Lissia, Amelia; Mingioni, Vindice; Pirino, Salvatore; Tanda, Francesco; Sanna, Maria Paola. - In: ATTI ... CONVEGNO NAZIONALE AIPVET. - ISSN 1825-2265. - (2005), pp. 19-22. (Intervento presentato al convegno 2. Congresso nazionale A.I.P.Vet.: atti).
Abstract:
The aim of this work was the detection of MAP in human with CD and in ovine with natural and experimental PTBC,
by in “situ” techniques. ISH showed strong signals in the macrophages infiltrating the mucosal and submucosal layers
in PTBC samples and positive signals in macrophages of CD cases. Furthermore, ISPCR signals were more numerous
than those obtained by ISH. This result indicate that ISPCR is able to detect also less copies of MAP DNA.
by in “situ” techniques. ISH showed strong signals in the macrophages infiltrating the mucosal and submucosal layers
in PTBC samples and positive signals in macrophages of CD cases. Furthermore, ISPCR signals were more numerous
than those obtained by ISH. This result indicate that ISPCR is able to detect also less copies of MAP DNA.
Tipologia CRIS:
4.2 Abstract in Atti di convegno
Keywords:
Paratuberculosis; Crohn’s disease; MAP;in situhybridization (ISH);in situPCR (ISPCR)
Elenco autori:
Rocca, Stefano; Leoni, Antonio; Sechi, Leonardo Antonio; Farigu, Serafina; Lissia, Amelia; Mingioni, Vindice; Pirino, Salvatore; Tanda, Francesco; Sanna, Maria Paola
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