The Diagnosis ofEchinococcus granulosusin dogs

The problem of diagnosingEchinococcus granulosusin dogs has still only been partially
resolved, even after the advent of biotechnology. The eggs of taeniid Cestoda are extremely similar, and
thus identification by microscopic examination of the faeces is risky and non-specific. For this reason,Echinococcus granulosuswas traditionally diagnosed in dogs ante mortem after an arecoline hydrobromate
purge. The faeces were examined macro and microscopically to establish if the adult tapeworm or
its proglottids were present. Although this method is 100% specific, it is bio-hazardous and time-consuming,
requires trained personnel, and its sensitivity varies. In the 1990s copro-antigens were discovered
and characterised. These are released by the adult worm in the faeces. This made it possible to use
enzyme-linked immune-adsorbent assay (ELISA) for in vitam diagnosis ofEchinococcus granulosus. In
recent years several PCR protocols have been published on the identification ofEchinococcus granulosusDNA from eggs or from adult parasites and new ways of diagnosing this cestode have been developed.