A new sensitive reversed phase high-performance liquid chromatography method for the quantitative determination of metoclopramide in canine plasma

A specific and sensitive high-performance liquid chromatographic method was developed for the determination of metoclopramide in canine plasma. The procedure involves fast liquid-liquid extraction and analysis on an octadecyl silane (ODS) column. A preliminary pharmacokinetic study was performed by applying the developed method to a single oral administration of metoclopramide (MCP) to a dog. The validation method yielded good results regarding linearity, precision, accuracy, and specificity. The procedure is suitable for separation and quantification of MCP in canine plasma, enough to quantify 0.2 ng/ml when 0.5 ml of plasma is used. This assay procedure might be useful for the pharmacokinetic study of MCP in dogs.