Data di Pubblicazione:
2017
Citazione:
Analysis of Sardinian myrtle (Myrtus communis L.) germplasm selections by SSR markers / Corona, Lucia; Mele, Caterina; Chessa, Innocenza; Mulas, Maurizio. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - 1172:(2017), pp. 165-170. [10.17660/ActaHortic.2017.1172.32]
Abstract:
Myrtus communis L., an evergreen shrub of the Mediterranean maquis, is widely
used for its medicinal and aromatic properties due to the essential oil content of its
leaves and fruits. It is also used as an ornamental plant and as a cut foliage crop. In
Sardinia, myrtle leaves and berries are used for the production of traditional myrtle
liqueurs. In this study, we report the use of simple sequence repeats (SSR) markers to
assess the genetic diversity among some Sardinian myrtle cultivars grown at the
“Antonio Milella" Experimental Station located in Oristano (Sardinia, Italy). Forty
cultivars were sampled from this collection, resulting from a morphology-based
selection program initiated in 1995. Genomic DNA was extracted from young leaves
using a commercial kit, and used as template for PCR analysis with a set of 14 primer
pairs. Fluorescently labeled amplification products were run on a capillary sequencer.
Genetic structure was investigated using the Bayesian clustering model implemented
in the STRUCTURE 2.3.3 software. The SSR markers used in this study identified
genetic variability among the studied myrtle cultivars and can be helpful in the
selection of material for obtaining new commercial cultivars and for implementing
myrtle preservation programs.
used for its medicinal and aromatic properties due to the essential oil content of its
leaves and fruits. It is also used as an ornamental plant and as a cut foliage crop. In
Sardinia, myrtle leaves and berries are used for the production of traditional myrtle
liqueurs. In this study, we report the use of simple sequence repeats (SSR) markers to
assess the genetic diversity among some Sardinian myrtle cultivars grown at the
“Antonio Milella" Experimental Station located in Oristano (Sardinia, Italy). Forty
cultivars were sampled from this collection, resulting from a morphology-based
selection program initiated in 1995. Genomic DNA was extracted from young leaves
using a commercial kit, and used as template for PCR analysis with a set of 14 primer
pairs. Fluorescently labeled amplification products were run on a capillary sequencer.
Genetic structure was investigated using the Bayesian clustering model implemented
in the STRUCTURE 2.3.3 software. The SSR markers used in this study identified
genetic variability among the studied myrtle cultivars and can be helpful in the
selection of material for obtaining new commercial cultivars and for implementing
myrtle preservation programs.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
breeding, domestication, molecular markers, population genetics, population structure
Elenco autori:
Corona, Lucia; Mele, Caterina; Chessa, Innocenza; Mulas, Maurizio
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